7BAG
C3b in complex with CP40
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.508, 90.528, 140.093 |
| Unit cell angles | 90.00, 108.84, 90.00 |
Refinement procedure
| Resolution | 47.820 - 2.000 |
| R-factor | 0.1957 |
| Rwork | 0.194 |
| R-free | 0.22500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wii |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.3.8) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.030 | 48.030 | 2.030 |
| High resolution limit [Å] | 2.000 | 10.950 | 2.000 |
| Rmerge | 0.097 | 0.049 | 1.961 |
| Rmeas | 0.111 | 0.056 | 2.243 |
| Rpim | 0.054 | 0.028 | 1.077 |
| Total number of observations | 666117 | 3928 | 33091 |
| Number of reflections | 159033 | 1025 | 7747 |
| <I/σ(I)> | 8.7 | 26.2 | 0.8 |
| Completeness [%] | 98.3 | 97.5 | 97.4 |
| Redundancy | 4.2 | 3.8 | 4.3 |
| CC(1/2) | 0.996 | 0.994 | 0.373 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 291 | 6.66% w/v polyethylene glycol 8000, 66.6 mM sodium chloride and 33.3 mM disodium phosphate/citric acid pH 4.0 |
