7B26
CirpA1 in complex with pseudo-monomeric Properdin lacking TSR2-3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.915890 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 166.310, 54.512, 70.581 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.710 - 3.400 |
R-factor | 0.2315 |
Rwork | 0.229 |
R-free | 0.27410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | CirpA1 6s08 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18rc4_3812) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.155 | 3.277 |
High resolution limit [Å] | 2.842 | 2.842 |
Rmerge | 1.820 | |
Number of reflections | 7444 | 372 |
<I/σ(I)> | 6.1 | |
Completeness [%] | 89.4 | |
Redundancy | 10.7 | |
CC(1/2) | 0.994 | 0.599 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.15 M Potassium thiocyanate, 0.1 M Tris, pH 7.5, 18 % w/v PEG 5000 MME |