7AZN
Structure of mouse AsterC (GramD1c) with a new cholesterol-derived compound
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2019-12-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.976 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 65.995, 49.397, 61.691 |
Unit cell angles | 90.00, 100.07, 90.00 |
Refinement procedure
Resolution | 26.770 - 2.090 |
R-factor | 0.2193 |
Rwork | 0.217 |
R-free | 0.25960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gqf |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.3) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.770 | 2.150 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.060 | 0.252 |
Rmeas | 0.083 | 0.347 |
Rpim | 0.057 | 0.239 |
Number of reflections | 11465 | 808 |
<I/σ(I)> | 7.1 | 2.9 |
Completeness [%] | 97.9 | 84.2 |
Redundancy | 3.2 | 2.7 |
CC(1/2) | 0.994 | 0.944 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 0.1 M SPG buffer pH 4 25 % PEG 1500 |