7AXX

Structure of WDR5:CS-VIP8 crystal after illumination at 405 nm and room temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	46.030, 46.900, 126.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.010 - 1.790
R-factor	0.16817
Rwork	0.166
R-free	0.21361
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iam
RMSD bond length	0.012
RMSD bond angle	1.530
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.010	1.859
High resolution limit [Å]	1.790	1.790
Rmerge	0.085	0.619
Rmeas	0.093	0.750
Rpim	0.037	0.411
Number of reflections	25827	6956
<I/σ(I)>	11.32	1.44
Completeness [%]	96.1	66.56
Redundancy	5.9	3.1
CC(1/2)	0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277.15	10% (w/v) PEG20000, 20% (v/v) PEG550 MME, 0.02 M sodium formate, 0.02 M ammonium acetate, 0.02 M trisodium citrate, 0.02 M sodium potassium L-tartrate, 0.02 M sodium oxamate