7AUW
Inhibitory complex of human meprin beta with mouse fetuin-B.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 126.660, 88.240, 156.660 |
| Unit cell angles | 90.00, 113.82, 90.00 |
Refinement procedure
| Resolution | 76.260 - 2.800 |
| R-factor | 0.2366 |
| Rwork | 0.236 |
| R-free | 0.26070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gwm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.300 | 2.970 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 73323 | 73323 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 93.6 | 94.9 |
| Redundancy | 6.9 | |
| CC(1/2) | 0.998 | 0.573 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.3 | 293 | 20.0% PEG smear medium, 0.1M ammonium sulfate, 0.05M magnesium sulfate heptahydrate, 0.1M bicine pH 9.3, 10% ethylene glycol. |
