7AU5
Tubulin-noscapine-analogue-14e complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-05-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 0.99999 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 104.642, 157.838, 180.350 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.880 - 2.200 |
R-factor | 0.1793 |
Rwork | 0.178 |
R-free | 0.21230 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 5lxt |
RMSD bond length | 0.002 |
RMSD bond angle | 0.512 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.880 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.079 | 2.003 |
Rmeas | 0.082 | 2.103 |
Number of reflections | 149600 | 14004 |
<I/σ(I)> | 24.5 | 1.09 |
Completeness [%] | 98.6 | 93.26 |
Redundancy | 13.2 | 10.8 |
CC(1/2) | 1.000 | 0.417 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 298 | 4 % PEG 4K, 7 % glycerol, 30 mM MgCl2, 30 mM CaCl2, 5 mM L-tyrosine and 100 mM MES/Imidazole pH 6.5 |