7ATS
The LIMK1 Kinase Domain Bound To LIJTF500127
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-03 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 81.096, 81.096, 173.953 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.710 - 2.800 |
R-factor | 0.2511 |
Rwork | 0.248 |
R-free | 0.31220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5nxc |
RMSD bond length | 0.011 |
RMSD bond angle | 1.343 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (7.0.053) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.710 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.061 | 0.539 |
Number of reflections | 8956 | 1255 |
<I/σ(I)> | 16.5 | 2.2 |
Completeness [%] | 100.0 | |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350 0.1 M bis-tris-propane pH 7.5 0.2 M potassium thiocyanate 10% ethylene glycol |