7ASS
OXA-48_L67F_CAZ. What Doesnt Kill You Makes You Stronger: Sub-MIC Selection Drives Cryptic Evolution of OXA-48
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9184 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 88.555, 108.476, 125.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.560 - 1.910 |
R-factor | 0.1894 |
Rwork | 0.187 |
R-free | 0.22980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5qb4 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.028 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.940 |
High resolution limit [Å] | 1.910 | 1.910 |
Rmerge | 0.165 | 1.870 |
Rpim | 0.099 | 1.110 |
Number of reflections | 94247 | 4653 |
<I/σ(I)> | 9.6 | 1.1 |
Completeness [%] | 100.0 | |
Redundancy | 7.1 | |
CC(1/2) | 0.997 | 0.583 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M Tris, pH 9.0, 28-30% PEG mono ethylene ether 500 |