7AMG
IL-17A in complex with small molecule modulators
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-03-04 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97949 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.006, 119.043, 54.290 |
| Unit cell angles | 90.00, 90.54, 90.00 |
Refinement procedure
| Resolution | 28.860 - 3.180 |
| R-factor | 0.20461 |
| Rwork | 0.204 |
| R-free | 0.22704 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hr9 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.042 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.870 | 3.400 |
| High resolution limit [Å] | 3.180 | 3.180 |
| Rmerge | 0.098 | 1.184 |
| Number of reflections | 17232 | 2973 |
| <I/σ(I)> | 9.2 | |
| Completeness [%] | 97.8 | |
| Redundancy | 2.9 | |
| CC(1/2) | 0.997 | 0.338 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG 10000, isopropanol, sodium citrate |
