7ALJ
Structure of Drosophila Notch EGF domains 11-13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-30 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.820, 31.286, 21.795 |
| Unit cell angles | 90.00, 90.77, 90.00 |
Refinement procedure
| Resolution | 45.200 - 1.520 |
| R-factor | 0.2034 |
| Rwork | 0.201 |
| R-free | 0.24140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vj3 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.446 |
| Data reduction software | xia2 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (dev_3965) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.200 | 1.577 |
| High resolution limit [Å] | 1.520 | 1.523 |
| Number of reflections | 18808 | 1771 |
| <I/σ(I)> | 7.75 | 1.68 |
| Completeness [%] | 98.4 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.996 | 0.701 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M MES 6.5, 1.8M Ammonium sulphate, 0.01M Cobalt chloride |
