7ALC

human GCH-GFRP stimulatory complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	C 1 2 1
Unit cell lengths	163.853, 113.922, 113.910
Unit cell angles	90.00, 130.21, 90.00

Refinement procedure

Resolution	86.990 - 1.726
R-factor	0.1979
Rwork	0.197
R-free	0.22730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1is8
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.7 (18-SEP-2020))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.990	1.934
High resolution limit [Å]	1.726	1.726
Number of reflections	162888	44799
<I/σ(I)>	7.9
Completeness [%]	99.1	99.7
Redundancy	3.5
CC(1/2)	0.998	0.260

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293.15	2 M NaCl; 0.1 M NaCit pH 6.0