7ALC
human GCH-GFRP stimulatory complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-12 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 163.853, 113.922, 113.910 |
| Unit cell angles | 90.00, 130.21, 90.00 |
Refinement procedure
| Resolution | 86.990 - 1.726 |
| R-factor | 0.1979 |
| Rwork | 0.197 |
| R-free | 0.22730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1is8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.980 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.7 (18-SEP-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.990 | 1.934 |
| High resolution limit [Å] | 1.726 | 1.726 |
| Number of reflections | 162888 | 44799 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 99.1 | 99.7 |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293.15 | 2 M NaCl; 0.1 M NaCit pH 6.0 |
