7AKK
Structure of a complement factor-receptor complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-07-21 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.98001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 111.290, 150.730, 111.300 |
| Unit cell angles | 90.00, 92.80, 90.00 |
Refinement procedure
| Resolution | 53.775 - 3.395 |
| Rwork | 0.192 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | wwPDB 2A74/2I07/4M76 |
| Data reduction software | XDS (Jan 31, 2020 BUILT=20200131) |
| Data scaling software | DIALS (3.3) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.775 | 3.520 |
| High resolution limit [Å] | 3.340 | 3.390 |
| Rmerge | 0.338 | 2.422 |
| Rmeas | 0.398 | 2.847 |
| Rpim | 0.209 | 1.486 |
| Number of reflections | 50383 | 4854 |
| <I/σ(I)> | 4.03 | 0.53 |
| Completeness [%] | 99.6 | 96.71 |
| Redundancy | 3.6 | 3.6 |
| CC(1/2) | 0.973 | 0.167 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100 mM Tris-HCl (pH 8.0), 8% (w/v) polyethylene glycol (PEG) 8000 |
