7AK5
Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.17 |
| Software | UCSF Chimera,PHENIX,RELION,Gctf,ISOLDE,Coot |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
