7AK5
Cryo-EM structure of respiratory complex I in the deactive state from Mus musculus at 3.2 A
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K2 QUANTUM (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.17 |
Software | UCSF Chimera,PHENIX,RELION,Gctf,ISOLDE,Coot |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |