7AEW
14-3-3 sigma bound to bis-phosphorylated aminopeptidase N (APN, CD13) via canonical and non-canonical binding motifs
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-29 |
Detector | DECTRIS EIGER2 XE 16M |
Wavelength(s) | 0.911650 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 82.210, 112.060, 62.610 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.516 - 1.200 |
Rwork | 0.162 |
R-free | 0.18360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mhr |
RMSD bond length | 0.015 |
RMSD bond angle | 1.876 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.520 | 1.300 |
High resolution limit [Å] | 1.200 | 1.200 |
Number of reflections | 86519 | 16344 |
<I/σ(I)> | 19.82 | |
Completeness [%] | 95.8 | |
Redundancy | 13.4 | |
CC(1/2) | 1.000 | 0.854 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES pH 7.7 0.19 M CaCl2 5% glycerol 27 % PEG400 |