7AD7

Crystal structure of human complement C5 in complex with the K8 bovine knob domain peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-28
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 21
Unit cell lengths	81.559, 161.695, 187.380
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	93.690 - 2.300
R-factor	0.2036
Rwork	0.202
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cu7
RMSD bond length	0.003
RMSD bond angle	0.627
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	REFMAC (5.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	93.690	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.043	0.361
Number of reflections	171135	10933
<I/σ(I)>	13.17	2.07
Completeness [%]	99.8	99.82
Redundancy	2
CC(1/2)	0.998	0.668

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		296	0.1M ADA, 14% ethanol.