7A5A
Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Monoclinic Crystal Form)
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-08 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 76.971, 108.260, 223.535 |
| Unit cell angles | 90.00, 93.22, 90.00 |
Refinement procedure
| Resolution | 49.050 - 2.989 |
| Rwork | 0.204 |
| R-free | 0.23950 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7a59 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.598 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.600 | 49.600 | 3.050 |
| High resolution limit [Å] | 2.989 | 14.640 | 2.990 |
| Rmerge | 0.184 | 0.051 | 1.411 |
| Rmeas | 0.198 | 0.056 | 1.544 |
| Rpim | 0.075 | 0.023 | 0.616 |
| Total number of observations | 3739 | 24431 | |
| Number of reflections | 73919 | 661 | 4043 |
| <I/σ(I)> | 7.8 | 26.1 | 1.2 |
| Completeness [%] | 99.3 | 96.8 | 89.2 |
| Redundancy | 7 | 5.7 | 6 |
| CC(1/2) | 0.995 | 0.997 | 0.555 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 0.5 uL of 13.4 mg/mL protein in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.5 uL of reservoir solution containing 0.1 M HEPES pH 7.5, 10% (w/v) PEG 8K, 8% (v/v) ethylene glycol |
