7A59
Crimean-Congo Hemorrhagic Fever Virus Envelope Glycoprotein Gc W1191H/W1197A/W1199A Mutant in Postfusion Conformation (Orthorhombic Crystal Form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-10 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.044 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 69.961, 216.083, 274.283 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.910 - 2.197 |
Rwork | 0.161 |
R-free | 0.19420 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.827 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | AutoSol |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.370 | 49.370 | 2.230 |
High resolution limit [Å] | 2.197 | 12.030 | 2.197 |
Rmerge | 0.071 | 0.032 | 1.167 |
Rmeas | 0.076 | 0.035 | 1.256 |
Rpim | 0.028 | 0.013 | 0.459 |
Number of reflections | 105875 | 732 | 4975 |
<I/σ(I)> | 15.4 | ||
Completeness [%] | 99.8 | 98.4 | 95.8 |
Redundancy | 7.1 | 6.7 | 7.2 |
CC(1/2) | 0.999 | 0.999 | 0.674 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291.15 | 0.75 uL of 12 mg/mL protein in 20 mM Tris-Cl pH 8.0 and 150 mM NaCl were added to 0.50 uL of reservoir solution containing 0.1 M MES pH 6.5, 0.1 M MgCl2 and 30% (v/v) PEG 400 |