7A3Q

Crystal structure of dengue 4 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.254
Spacegroup name	P 1 21 1
Unit cell lengths	65.985, 133.180, 93.536
Unit cell angles	90.00, 92.15, 90.00

Refinement procedure

Resolution	20.000 - 2.700
R-factor	0.2171
Rwork	0.216
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3uaj 3fku
RMSD bond length	0.007
RMSD bond angle	0.930
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.800
High resolution limit [Å]	2.700	2.700
Number of reflections	43980	4614
<I/σ(I)>	12.5	1.2
Completeness [%]	99.1	99.1
Redundancy	3.19
CC(1/2)	0.998	0.688

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	100 mM CAPSO pH 9.6, 16.7% (w/v) PEG 8,000, 200 mM NaCl