7A3O

Crystal structure of dengue 1 virus envelope glycoprotein in complex with the scFv fragment of the broadly neutralizing human antibody EDE1 C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 63 2 2
Unit cell lengths	135.555, 135.555, 160.268
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.760 - 2.800
R-factor	0.2207
Rwork	0.218
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ut9
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.800	2.800
Number of reflections	21987	3110
<I/σ(I)>	14.5
Completeness [%]	99.6	98.8
Redundancy	8.2
CC(1/2)	0.999	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.5	291	50 mM Tris pH 8.5, 25% PEG 3,350, 200 mM (NH4)2SO4, 1.2% myo-inositol