7A3N
Crystal structure of Zika virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE1 C10
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9870 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 53.964, 168.610, 220.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.580 | 2.040 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.664 | |
Number of reflections | 68764 | 3874 |
<I/σ(I)> | 5.3 | |
Completeness [%] | 98.9 | |
Redundancy | 7.8 | |
CC(1/2) | 0.954 | 0.111 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 100 mM Imidazole (pH 8), 10% PEG 8,000, 200 mM CaCl2 |