7A38
Crystal structure of the c-Src SH3 domain mutant V111L-N113S-T114S-Q128E at pH 6.0
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.277, 40.437, 38.606 |
Unit cell angles | 90.00, 100.35, 90.00 |
Refinement procedure
Resolution | 37.980 - 1.620 |
R-factor | 0.1736 |
Rwork | 0.172 |
R-free | 0.19850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jz4 |
Data reduction software | XDS |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.980 | 37.980 | 1.700 |
High resolution limit [Å] | 1.620 | 5.110 | 1.620 |
Rmerge | 0.088 | 0.047 | 0.612 |
Rmeas | 0.097 | 0.052 | 0.676 |
Rpim | 0.040 | 0.022 | 0.283 |
Total number of observations | 70827 | 2252 | 9951 |
Number of reflections | 12623 | 425 | 1815 |
<I/σ(I)> | 10.7 | 29.4 | 2.3 |
Completeness [%] | 99.6 | 99.5 | 99.3 |
Redundancy | 5.6 | 5.3 | 5.5 |
CC(1/2) | 0.998 | 0.998 | 0.912 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 298 | 1.5M ammonium sulfate, 0.1M MES, 10% glycerol |