7ZZO
HDAC2 in complex with an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-16 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 92.079, 97.444, 139.049 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.800 - 2.000 |
| R-factor | 0.1743 |
| Rwork | 0.172 |
| R-free | 0.22500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.327 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.800 | 2.060 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmeas | 0.133 | 0.758 |
| Number of reflections | 80136 | 5445 |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 94.6 | 73.9 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 38% PEG 300 0.1M pH=8.8 CHES/NaOH |
