7ZVN
Crystal structure of human Annexin A2 in complex with full phosphorothioate 5-10 2'-methoxyethyl DNA gapmer antisense oligonucleotide solved at 1.87 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P13 (MX1) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.621, 57.502, 70.478 |
| Unit cell angles | 90.00, 90.24, 90.00 |
Refinement procedure
| Resolution | 43.750 - 1.870 |
| R-factor | 0.1688 |
| Rwork | 0.167 |
| R-free | 0.19810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5lpu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.026 |
| Data reduction software | XDS (v3) |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.750 | 1.990 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Number of reflections | 36723 | 35540 |
| <I/σ(I)> | 12.14 | |
| Completeness [%] | 99.8 | |
| Redundancy | 6.6 | |
| CC(1/2) | 1.000 | 0.720 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.3 | 290 | 4% w/v PEG 4000, 14% v/v glycerol, 0.03 M MgCl2/CaCl2, 0.1 M bicine/Trizma base pH 8.3 |
