7ZSC

Crystal structure of the heterodimeric human C-P4H-II with truncated alpha subunit (C-P4H-II delta281)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	H 3
Unit cell lengths	252.879, 252.879, 89.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.690 - 3.850
R-factor	0.2472
Rwork	0.244
R-free	0.27780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jig 4el1 6i7s
RMSD bond length	0.008
RMSD bond angle	0.980
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.690	3.990
High resolution limit [Å]	3.850	3.850
Rmerge	0.064	1.862
Rmeas	0.071	2.058
Rpim	0.031	0.873
Number of reflections	20176	1969
<I/σ(I)>	8.2	0.7
Completeness [%]	99.9	100
Redundancy	5.3	5.5
CC(1/2)	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	278	20% (w/v) PEG4000, 0.2 M lithium sulfate, 0.1 M MES, 3% methanol