7ZRB
Crystal structure of Beta-catenin Armadillo repeats domain in complex with the inhibitor RS6452
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ELETTRA BEAMLINE 11.2C |
Synchrotron site | ELETTRA |
Beamline | 11.2C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-02 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.880, 160.240, 79.970 |
Unit cell angles | 90.00, 99.63, 90.00 |
Refinement procedure
Resolution | 45.264 - 3.434 |
R-factor | 0.2359 |
Rwork | 0.233 |
R-free | 0.28330 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1th1 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.653 |
Data reduction software | XDS |
Data scaling software | SAINT |
Phasing software | AMoRE |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.840 | 3.660 |
High resolution limit [Å] | 3.430 | 3.430 |
Rmerge | 0.219 | 1.882 |
Number of reflections | 12910 | 646 |
<I/σ(I)> | 7.3 | 1 |
Completeness [%] | 90.8 | |
Redundancy | 3.4 | |
CC(1/2) | 0.996 | 0.358 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | 7-9% (w/v) PEG 6000, 100 mM Na Citrate, pH 5.6, 5 mM DTT, 15% (v/v) Isopropanol |