7ZLM
Crystal structure of SOCS2:ElonginB:ElonginC in complex with compound MN551
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-26 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.785, 172.879, 185.809 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 78.370 - 1.790 |
| R-factor | 0.1907 |
| Rwork | 0.189 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SBC |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.937 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.370 | 1.820 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 1.605 | |
| Number of reflections | 181660 | 8234 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.991 | 0.351 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2 M Potassium sodium tartrate tetrahydrate, 0.1 M Bis-Tris propane 6.5, 20 % w/v PEG 3350 with seeding |
