7ZDD

Crystal structure of TRIM33 PHD-Bromodomain isoform B in complex with H3K10ac histone peptide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	197
Detector technology	PIXEL
Collection date	2021-07-05
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	36.130, 52.893, 105.698
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.380 - 1.625
R-factor	0.2099
Rwork	0.208
R-free	0.25020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mr8
RMSD bond length	0.008
RMSD bond angle	0.860
Data reduction software	autoPROC (1.1.7 (20200918))
Data scaling software	STARANISO (Version 2.3.46 (17-Sep-2020)
Phasing software	PHASER (2.8.3)
Refinement software	BUSTER (2.11.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.385	1.776
High resolution limit [Å]	1.625	1.625
Rmerge	0.098	1.184
Rpim	0.043	0.462
Number of reflections	16564	829
<I/σ(I)>	12	1.6
Completeness [%]	92.2	57.7
Redundancy	6.2	7.3
CC(1/2)	0.998	0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	295.15	0.2 M ammonium chloride, 0.1 M sodium acetate pH 5.0, 20% w/v PEG 6000