7Z5M
Crystal structure of YTHDF2 with compound YLI_DC1_008
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-05-16 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 80.330, 80.330, 114.030 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.100 - 1.700 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.23310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rdn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.825 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.100 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 46140 | 7396 |
| <I/σ(I)> | 45.99 | |
| Completeness [%] | 99.9 | |
| Redundancy | 12.6 | |
| CC(1/2) | 1.000 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 4.9 |
