7Z3P
Crystal structure of the mouse leptin:LepR-CRH2 encounter complex to 1.95 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-02-01 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980110 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.727, 52.811, 89.055 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.350 - 1.943 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ax8 3v6o |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS (version March 15, 2019) |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER (2.7.0) |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.350 | 1.990 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmeas | 0.127 | 3.271 |
| Number of reflections | 34115 | 2017 |
| <I/σ(I)> | 16.08 | 0.8 |
| Completeness [%] | 99.2 | 88.9 |
| Redundancy | 13.2 | |
| CC(1/2) | 0.999 | 0.346 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | BCS screen, condition H1 0.04 M Calcium chloride dihydrate 0.04 M sodium formate 0.1 M Tris pH 8.0 25% PEG Smear Low cryo: 20 % PEG 400 |
