7Z26
Crystal structure of YTHDF2 YTH domain in complex with m6A RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-08-28 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1 |
Spacegroup name | P 65 |
Unit cell lengths | 80.690, 80.690, 114.270 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.340 - 1.900 |
R-factor | 0.1989 |
Rwork | 0.197 |
R-free | 0.23020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4rdn |
RMSD bond length | 0.008 |
RMSD bond angle | 1.058 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20_4459) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.340 | 2.010 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 33384 | 5365 |
<I/σ(I)> | 20.97 | |
Completeness [%] | 99.9 | |
Redundancy | 9.06 | |
CC(1/2) | 0.999 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2.025 M Ammonium sulfate, 0.1 M Sodium citrate tribasic pH 5.2 |