7Z21
BAF A12T bound to the lamin A/C Ig-fold domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-10 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.965459 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.110, 75.073, 65.034 |
| Unit cell angles | 90.00, 114.19, 90.00 |
Refinement procedure
| Resolution | 14.900 - 1.629 |
| R-factor | 0.27 |
| Rwork | 0.268 |
| R-free | 0.30040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ghd |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.950 |
| Data reduction software | XDS (VERSION Jan 10, 2022 BUILT=20220110) |
| Data scaling software | STARANISO (2.3.77) |
| Phasing software | MOLREP (11.7.03) |
| Refinement software | BUSTER (2.10.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.540 | 1.670 |
| High resolution limit [Å] | 1.630 | 1.630 |
| Rmerge | 0.142 | 0.993 |
| Rmeas | 0.172 | 1.209 |
| Rpim | 0.097 | 0.680 |
| Number of reflections | 68861 | 5048 |
| <I/σ(I)> | 5.6 | 1 |
| Completeness [%] | 99.2 | 99 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.992 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 3 M ammonium sulfate 0.1 M bicine pH 9 |
