7Z21

BAF A12T bound to the lamin A/C Ig-fold domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-10
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.965459
Spacegroup name	P 1 21 1
Unit cell lengths	63.110, 75.073, 65.034
Unit cell angles	90.00, 114.19, 90.00

Refinement procedure

Resolution	14.900 - 1.629
R-factor	0.27
Rwork	0.268
R-free	0.30040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ghd
RMSD bond length	0.007
RMSD bond angle	0.950
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220110)
Data scaling software	STARANISO (2.3.77)
Phasing software	MOLREP (11.7.03)
Refinement software	BUSTER (2.10.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.540	1.670
High resolution limit [Å]	1.630	1.630
Rmerge	0.142	0.993
Rmeas	0.172	1.209
Rpim	0.097	0.680
Number of reflections	68861	5048
<I/σ(I)>	5.6	1
Completeness [%]	99.2	99
Redundancy	3	3
CC(1/2)	0.992	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	3 M ammonium sulfate 0.1 M bicine pH 9