7Z0Q
MHC-II dynamics are maintained in HLA-DR allotypes to ensure catalyzed peptide exchange
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-09-30 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91841 |
Spacegroup name | H 3 |
Unit cell lengths | 134.094, 134.094, 72.178 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.710 - 2.100 |
R-factor | 0.216 |
Rwork | 0.215 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x5x |
RMSD bond length | 0.010 |
RMSD bond angle | 0.720 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.710 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmeas | 0.076 | 2.559 |
Number of reflections | 28123 | 2850 |
<I/σ(I)> | 12.06 | |
Completeness [%] | 98.6 | |
Redundancy | 4 | |
CC(1/2) | 0.999 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.15M Sodiummalonate |