7YXC
Crystal structure of WT AncGR2-LBD bound to dexamethasone and SHP coregulator fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9788 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 86.605, 52.435, 69.600 |
| Unit cell angles | 90.00, 116.62, 90.00 |
Refinement procedure
| Resolution | 43.420 - 2.250 |
| R-factor | 0.195 |
| Rwork | 0.193 |
| R-free | 0.24520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ufs |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.565 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.420 | 2.320 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmeas | 0.069 | 0.697 |
| Rpim | 0.039 | 0.439 |
| Number of reflections | 12530 | 907 |
| <I/σ(I)> | 11.1 | 1.8 |
| Completeness [%] | 93.8 | 75.5 |
| Redundancy | 2.7 | 2.2 |
| CC(1/2) | 0.997 | 0.654 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293.15 | 85 mM sodium cacodylate trihydrate, pH 6.5, 0.17 M sodium acetate trihydrate, 25.5% (w/v) PEG8000, 15% (v/v) glycerol |
