7YSE

Crystal structure of E. coli heterotetrameric GlyRS in complex with tRNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	P 2 2 21
Unit cell lengths	72.710, 162.123, 324.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.005 - 2.907
Rwork	0.230
R-free	0.25980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7eiv 4mgm
RMSD bond length	0.004
RMSD bond angle	1.231
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	162.120	3.060
High resolution limit [Å]	2.907	2.907
Rmerge	0.138	0.749
Rpim	0.059	0.304
Number of reflections	85473	12313
<I/σ(I)>	9.4
Completeness [%]	100.0
Redundancy	6.6
CC(1/2)	0.990	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	0.15M MgCl2, 0.1M NaCl, 0.1M Tris-HCl pH 8.5, 30% PEG 300