7YMF
Crystal Structure of DDX3X449_450ET>DP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9791 |
Spacegroup name | H 3 |
Unit cell lengths | 106.560, 106.560, 116.710 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.320 - 2.300 |
R-factor | 0.1881 |
Rwork | 0.185 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4pxa |
RMSD bond length | 0.012 |
RMSD bond angle | 1.375 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.113 | 0.698 |
Rpim | 0.039 | 0.264 |
Number of reflections | 21930 | 2050 |
<I/σ(I)> | 24.5 | 4.8 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 9.2 | 7.6 |
CC(1/2) | 0.980 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M MES pH 6.5, 1.5 M Sodium Formate , 50 mM Magnesium chloride |