7YDS

The structure of the bispecific antibody targeted PD-L1 and 4-1BB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-09
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54056
Spacegroup name	P 21 21 21
Unit cell lengths	71.076, 94.880, 96.069
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	13.140 - 2.300
R-factor	0.1968
Rwork	0.194
R-free	0.24610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6wkm 3bis
Data reduction software	CrysalisPro
Data scaling software	Aimless
Phasing software	MLPHARE
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	13.140	2.382
High resolution limit [Å]	2.300	2.300
Rmerge	0.109	0.582
Rmeas	0.154	0.823
Rpim	0.109	0.582
Number of reflections	29320	2885
<I/σ(I)>	8.23	1.29
Completeness [%]	99.3	99.55
Redundancy	2	2
CC(1/2)	0.983	0.455

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289.15	18% PEG4000, 0.1M Bis-tris pH 6.2