7XY8
Crystal structure of antibody Fab fragment in complex with CD147(EMMPIRIN)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-01-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 64.960, 93.370, 98.309 |
Unit cell angles | 90.00, 90.89, 90.00 |
Refinement procedure
Resolution | 35.910 - 2.300 |
R-factor | 0.238 |
Rwork | 0.236 |
R-free | 0.28090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3b5h |
RMSD bond length | 0.007 |
RMSD bond angle | 1.304 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.911 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.360 | |
Rmeas | 0.105 | 0.439 |
Rpim | 0.060 | 0.247 |
Number of reflections | 51301 | 7430 |
<I/σ(I)> | 7.8 | 2.1 |
Completeness [%] | 98.4 | 97.8 |
Redundancy | 3 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 293 | 0.1M Sodium Malonate pH 7.0, 12% (w/v) Polyethylene Glycol 3350 |