7XUZ
Crystal structure of a HDAC4-MEF2A-DNA ternary complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-10-09 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.987 |
Spacegroup name | P 65 |
Unit cell lengths | 93.210, 93.210, 224.920 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.361 - 3.591 |
R-factor | 0.2605 |
Rwork | 0.256 |
R-free | 0.29800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tqe 2h8n |
RMSD bond length | 0.002 |
RMSD bond angle | 0.450 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.363 | 3.730 |
High resolution limit [Å] | 3.590 | 3.590 |
Rmerge | 0.097 | 0.521 |
Number of reflections | 12749 | 1183 |
<I/σ(I)> | 19.51 | |
Completeness [%] | 98.0 | |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0 mM HEPES pH 7.5, 0.2 M NaCl, 3% (v/v) PEG 4000 |