7XTP
eIF4E in Complex with a Disulphide-Free Autonomous VH Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-15 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95373 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.623, 57.207, 78.963 |
| Unit cell angles | 90.00, 93.95, 90.00 |
Refinement procedure
| Resolution | 46.332 - 1.828 |
| Rwork | 0.226 |
| R-free | 0.25930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7d6y |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.332 | 1.890 |
| High resolution limit [Å] | 1.828 | 1.828 |
| Rmeas | 0.147 | 1.691 |
| Number of reflections | 59269 | 3593 |
| <I/σ(I)> | 11.8 | 1.3 |
| Completeness [%] | 99.2 | 97.5 |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1M Sodium HEPES pH7.5, 25% PEG 6000 |
