7XRC

Crystal Structure of the dimeric Brn2 (Pou3f2) POU domain bound to palindromic MORE DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	C 1 2 1
Unit cell lengths	94.350, 50.630, 69.310
Unit cell angles	90.00, 129.06, 90.00

Refinement procedure

Resolution	34.350 - 1.890
R-factor	0.2014
Rwork	0.200
R-free	0.22680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xsd
RMSD bond length	0.008
RMSD bond angle	1.188
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.080	2.000
High resolution limit [Å]	1.890	1.890
Number of reflections	19759	3185
<I/σ(I)>	20.15	1.04
Completeness [%]	95.7
Redundancy	6.96
CC(1/2)	1.000	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	291.15	10%PEG 4000,magnesium chloride