7XL1
Crystal structure of chimeric 7D12-Vob nanobody at 1.65 Angstrom
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-1A |
Synchrotron site | Photon Factory |
Beamline | BL-1A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-16 |
Detector | DECTRIS EIGER R 4M |
Wavelength(s) | 1.000 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 68.580, 68.580, 103.660 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.440 - 1.650 |
R-factor | 0.158 |
Rwork | 0.156 |
R-free | 0.20600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7xl0 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.431 |
Data reduction software | MOSFLM (7.2.2) |
Data scaling software | SCALA (3.3.22) |
Phasing software | PHASER (2.8.2) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.500 | 1.740 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.108 | 0.963 |
Rmeas | 0.327 | |
Rpim | 0.038 | 0.327 |
Number of reflections | 30042 | 4233 |
<I/σ(I)> | 12 | 2.3 |
Completeness [%] | 98.8 | 97.8 |
Redundancy | 8.7 | 9.2 |
CC(1/2) | 0.997 | 0.724 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M sodium malonate pH 4.0 20% PEG 3,350 |