7XJF

Crystal structure of 6MW3211 Fab in complex with CD47

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL19U1
Synchrotron site	NFPSS
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 21
Unit cell lengths	60.830, 100.480, 163.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.830 - 2.600
R-factor	0.1896
Rwork	0.187
R-free	0.23140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5tzu 6jjp
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.690
High resolution limit [Å]	2.600	5.600	2.600
Rmerge	0.129	0.057	1.010
Rmeas	0.136	0.061	1.064
Rpim	0.043	0.020	0.332
Total number of observations	311060
Number of reflections	31874	3409	3133
<I/σ(I)>	5.9
Completeness [%]	99.9	99.9	100
Redundancy	9.8	8.7	10.4
CC(1/2)		0.998	0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	289	2.2 M DL-malic acid, pH 5.5