7X8K

Arabidopsis GDP-D-mannose pyrophosphorylase (VTC1) structure (product-bound)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	H 3 2
Unit cell lengths	185.113, 185.113, 371.752
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.820 - 3.000
R-factor	0.2351
Rwork	0.234
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7d72
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.091
Rpim	0.031	0.366
Number of reflections	49084	4853
<I/σ(I)>	26	2.1
Completeness [%]	99.8	100
Redundancy	11.1	11
CC(1/2)	0.969	0.859

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	magnesium formate, PEG 3350