7X4N

Crystal Structure of C. elegans kinesin-4 KLP-12 complexed with tubulin and DARPin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	93
Detector technology	PIXEL
Collection date	2020-10-09
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	82.330, 80.980, 117.750
Unit cell angles	90.00, 93.51, 90.00

Refinement procedure

Resolution	49.250 - 2.880
R-factor	0.2149
Rwork	0.210
R-free	0.29580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5mio
RMSD bond length	0.010
RMSD bond angle	1.399
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.050
High resolution limit [Å]	2.880	2.880
Rmeas	0.355	1.970
Number of reflections	34669	5455
<I/σ(I)>	19.28	3.39
Completeness [%]	98.2
Redundancy	87.1
CC(1/2)	0.977	0.513

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	ammonium acetate, HEPES, polyethylene glycol (PEG) 3350