7WZX

The structure of a Twitch Radical SAM Dehydrogenase SpeY

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-01-30
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97846
Spacegroup name	P 65 2 2
Unit cell lengths	93.930, 93.930, 143.420
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.216 - 1.980
R-factor	0.210486832907
Rwork	0.209
R-free	0.23413
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7wzv
RMSD bond length	0.005
RMSD bond angle	0.950
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.630	2.030
High resolution limit [Å]	1.980	1.980
Rmerge		0.963
Number of reflections	26783	1844
<I/σ(I)>	42.3
Completeness [%]	100.0
Redundancy	37.9
CC(1/2)	1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.5 M Sodium cacodylate trihydrate (pH 6.0), 1.8 M Ammonium sulfate and 0.05 M Magnesium acetate tetrahydrate