7WZX

The structure of a Twitch Radical SAM Dehydrogenase SpeY

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	4Fe-4S cluster-binding domain-containing protein	polymer	322	36014.6	1	UniProt (A8WEZ7)	Streptomyces spectabilis	SpeY
2	B, C (A)	IRON/SULFUR CLUSTER	non-polymer		351.6	2	Chemie (SF4)
3	D (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
4	E, F, G, H (A)	GLYCEROL	non-polymer		92.1	4	Chemie (GOL)
5	I (A)	(2~{S},4~{S},6~{R})-2-[(2~{S},3~{R},5~{S},6~{R})-3,5-bis(methylamino)-2,4,6-tris(oxidanyl)cyclohexyl]oxy-6-methyl-4-oxidanyl-oxan-3-one	non-polymer		334.4	1	Chemie (7PK)
6	J, K (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
7	L (A)	water	water		18.0	81	Chemie (HOH)

Sequence modifications

A: 1 - 302 (UniProt: A8WEZ7)

PDB	External Database	Details
Met -19	-	initiating methionine
Gly -18	-	expression tag
Ser -17	-	expression tag
Ser -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
Ser -9	-	expression tag
Ser -8	-	expression tag
Gly -7	-	expression tag
Leu -6	-	expression tag
Val -5	-	expression tag
Pro -4	-	expression tag
Arg -3	-	expression tag
Gly -2	-	expression tag
Ser -1	-	expression tag
His 0	-	expression tag
Ala 269	Glu 269	conflict
Ala 270	Glu 270	conflict

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	36014.6
Non-Polymers*	Number of molecules	10
	Total formula weight	1996.6
All*	Total formula weight	38011.2

*Water molecules are not included.