7WVK

Crystal structure of human WDR5 in complex with compound 19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97915
Spacegroup name	C 2 2 21
Unit cell lengths	78.330, 98.380, 79.806
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.870 - 1.420
R-factor	0.1639
Rwork	0.163
R-free	0.18240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y7r
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.870	1.421
High resolution limit [Å]	1.416	1.416
Number of reflections	58768
<I/σ(I)>	18.9
Completeness [%]	99.9
Redundancy	8.2
CC(1/2)	0.999	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	0.1M Bis-Tris, 0.2 M NH4OAC, 30% PEG3350