7WG3

Structural basis of interleukin-17B receptor in complex with a neutralizing antibody D9 for guiding humanization and affinity maturation for cancer therapy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	110
Detector technology	CCD
Collection date	2018-04-18
Detector	RAYONIX MX300-HS
Wavelength(s)	1.007
Spacegroup name	P 1 21 1
Unit cell lengths	70.020, 195.030, 98.840
Unit cell angles	90.00, 98.13, 90.00

Refinement procedure

Resolution	38.030 - 2.190
R-factor	0.1954
Rwork	0.193
R-free	0.23790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hjg 4kuz 3jvf
RMSD bond length	0.008
RMSD bond angle	1.081
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.030	2.268
High resolution limit [Å]	2.190	2.190
Rmerge	0.056	0.508
Rmeas	0.079
Number of reflections	134260	13343
<I/σ(I)>	7.08
Completeness [%]	100.0
Redundancy	2
CC(1/2)	0.996	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.2	295	0.1M Bicine, pH 8.2, 17% PEG 3350