4KUZ

Crystal structure of anti-emmprin antibody 4A5 Fab in trigonal form

Summary for 4KUZ

• Entry DOI: 10.2210/pdb4kuz/pdb
• Descriptor: antibody 4A5 light chain, antibody 4A5 heavy chain, SULFATE ION, ... (4 entities in total)
• Functional Keywords: immunoglobulin fold, immune system
• Biological source: Mus musculus, Homo sapiens (mouse, human); More
• Total number of polymer chains: 2
• Total formula weight: 49079.46

Authors

Teplyakov, A.,Obmolova, G.,Gilliland, G.L. (deposition date: 2013-05-22, release date: 2014-03-26, Last modification date: 2024-11-20)

Primary citation

Teplyakov, A.,Obmolova, G.,Malia, T.J.,Luo, J.,Gilliland, G.L.
Structural evidence for a constrained conformation of short CDR-L3 in antibodies.
Proteins, 82:1679-1683, 2014

PubMed Abstract: Three Fab structures used as targets in the Antibody Modeling Assessment presented a challenge for modeling CDR-L3 due to deviations from the most typical canonical structure. In all three antibodies CDR-L3 has eight residues, which is one residue shorter than usual, and has a conformation that is rarely observed in crystal structures. We analyzed the sequence and structural determinants of this conformation and found that the "short" CDR-L3 is remarkably rigid and retains the conformation in the interactions with antigens and neighboring CDRs.

PubMed: 24470236
DOI: 10.1002/prot.24522

Experimental method

X-RAY DIFFRACTION (2.7 Å)