7W6A

Crystal structure of the MLL1 (N3861I/Q3867L/C3882SS)-RBBP5-ASH2L complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-07
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	75.639, 44.828, 119.141
Unit cell angles	90.00, 107.42, 90.00

Refinement procedure

Resolution	37.810 - 2.210
R-factor	0.1862
Rwork	0.184
R-free	0.22880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f6l
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.240
High resolution limit [Å]	2.200	5.970	2.200
Rmerge	0.136	0.062	0.650
Rmeas	0.151	0.069	0.747
Rpim	0.066	0.030	0.357
Total number of observations	97804
Number of reflections	18972	1018	868
<I/σ(I)>	4.4
Completeness [%]	98.9	99.9	91.8
Redundancy	5.2	5.2	4
CC(1/2)		0.995	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	0.2 M Sodium chloride, 0.1 M HEPES, pH 7.5 25% w/v polyethylene glycol 3350